Key Features

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Molecular Diagnostics Research Training

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Plant Tissue Culture

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Hands-on laboratory practice

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Best Researcher

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About us

Kalp Laboratories (A unit of Pvt. Ltd.) situated in Mathura city (Uttar Pradesh) is highly progressive biotechnology centre engaged in the Hands-On practice and basic skill development of young students in field of Life Science/ Biotechnology/ Microbiology/ Molecular Diagnostics Research Training. The primary purpose of a trainee/ researcher / student is getting a well equipped laboratory for doing the experiment that is to be reported.

So it is important to set the laboratory by establishing that learning perspective and fundamentals In life science, biotechnology and microbiology combined with extensive hands-on laboratory practice and instrumental learning skill.

Recognizing the wide spectrum of needs of approaching students and trainees,
Kalp Biotech Laboratory offers 1 month to 1 year Hands-on Industrial Training/ Project/ Dissertation Work and Summer & Winter Training Programs for the students of any field of Biotechnology/Life Sciences and
Medical Biotechnology.

Training Programme Detail

Biochemistry

1. Introduction to Good Laboratory Practices and General Safety Instructions
2. Instrumentation: Working with Autoclave, pH meter, Laminar Air Flow System, Incubator/ Shaker, Hot Air Oven, Microscope, Centrifuge, Spectrophotometer and Other
Instruments
3. Basics of Calculations; Buffers (Acetate, Citrate and Tris HCl buffer) and Reagent Preparation
4. Sterilization Techniques: Dry, Wet and Chemical Sterilization
5. Separation of chloroplasts by sucrose density gradient centrifugation
6. Protein isolation and quantification by Lowry and TCA methods
7. Higher absorption spectra(λmax) for proteins
8. Protein separation by SDS- PAGE and documentation
9. Western Blotting for protein identification
10. Determination of logic properties of amino acids by titrations
11. Separation of amino acid by paper chromatography
12. Separation of amino acid by paper thin layer chromatography
13. Separation of amino acid by paper thin layer chromatography
14. Isolation of secondary metabolites from various plants using solvents
15. Phytochemical analysis of plant secondary metabolites
16. Estimation of carbohydrates in plant samples by anthrone reagent
17. Determination of reducing sugar by Nelson Somagi method.
18. Casein purification from buffalo milk
19. pH determination of Soil samples
20. Estimation of citric acid production from Aspergillus niger

Molecular Biology

1. Isolation and Purification of Genomic DNA from Bacteria, plasmid and Plant samples
2. Quantification of Isolated Genomic DNA using Spectrophotometer
3. Agarose Gel Electrophoresis of Genomic DNA
4. Agarose Gel Electrophoresis of Isolated Plasmid DNA
5. RNA Isolation from Plant Sources
6. Purification & Quantification of RNA
7. Agarose Gel Electrophoresis of Isolated RNA
8. Polymerase Chain Reaction (PCR)
9. Primer Designing and Optimization of PCR
10. Gel Electrophoresis of PCR Products with Molecular Markers
11. Restriction Digestion of Genomic DNA
12. DNA profiling
13. Southern Blotting Technique (Transfer of DNA to Membrane)
14. Competent Cell Preparation of E. coli
15. Cloning of cDNA into Digested Plasmid (cDNA Ligation)

Microbiology & Tissue culture

1. Various Media (Solid/Liquid) preparation and Its Sterilization
2. Pure Culture Techniques (Streaking, Pouring & Plating, etc.)
3. Isolation and Screening of Microorganisms
4. Examine of Microbial contamination of food
5. Culturing & Sub Culturing Methods of Microbes
6. Isolation and Culturing of microbes from Soil Samples
7. Isolation and Culturing of microbes from Water Samples
8. Isolation and Culturing of microbes from Air Samples
9. Screening of Microbes (Staining Techniques)4

  • Gram Staining
  • Endospore Staining
  • Capsular Staining
  • Flagellar Staining

10.The sensitivity of bacteria towards different antibiotics
11. Optimization of Growth Condition for Industrially Important Microorganisms
12.Isolation and identification of enzyme producing microbe
13. Measurement of fungal growth by colony diameter
14. Measurement of bacterial growth by turbidity measurement
15. To study the Components, use and care of Compound Microscope
16. To study different stages of mitosis in cell division in permanent slides
17. Culture of embryos of crop seeds on MS media.
18. Culture of various explants

Enzymology & Environment

1. Estimation of enzyme activity in plant samples

  • Catalase activity
  • Ascorbate peroxidase
  • Glutathione reductase
  • Superoxide dismutase

2. Amylase Enzyme Assay
3. Degradation of pesticides in soil and estimation of its residues
4. Determination of fluoride in water sample.
5. Determination of biological oxygen demand (BOD) of water samples

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